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The formal paper should be 3-4 pages. Do not work together. The paper should address the following:
A new protein has been discovered in tumors. It is 157 amino acids long. It is implicated in disease. It is a NADPH dependent enzyme.
There is a specific mutant protein that changes two alanine to glutamic acids in the protein.
How will you purify the protein? How do you purify the mutant protein from the native WT protein.
Is it monomer? How do you know? Is it disulphide bonded? How will you determine the state of native cos residues?
Do the mutations in the protein affect the activity or quaternary structure of the protein? How will you determine the effects on activity?
Are there any other properties you can determine about the structure?
Please address the above questions with respect to your hypothetical protein.
Please refer to and use sources primarily from the lab manual attached, thanks
